تأثیر شش هفته تمرین استقامتی بر بیان پروتئین سینتافیلین در بافت نخاع موش‌های صحرایی مبتلا به نوروپاتی دیابتی تجربی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشگاه تهران، پردیس بین المللی کیش، ایران.

2 دانشیار، گروه فیزیولوژی ورزش، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران.

3 استاد، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه خوارزمی، تهران، ایران.

4 دانشیار، گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه ولی‌عصر (عج)، رفسنجان، ایران.

10.22123/chj.2022.282939.1716

چکیده

مقدمه: نوروپاتی از عوارض شایع دیابت شیرین است. پروتئین سینتافیلین (Syntaphilin) در فرآیند لنگرکردن (Anchoring) میتوکندری با میکروتوبول­ها نقش دارد. هدف از پژوهش حاضر، تعیین تأثیر شش هفته تمرین استقامتی بر بیان پروتئین سینتافیلین در بافت نخاع موش­های صحرایی مبتلا به نوروپاتی دیابتی تجربی بود.
مواد و روش‌ها: 40 سر موش صحرایی به صورت تصادفی به چهار گروه تقسیم شدند (دیابتی کنترل (DC)- دیابتی تمرین (DT)- کنترل سالم (HC)- تمرین سالم (HT)). القای دیابت با تزریق درون صفاقی محلول استرپتوزوسین (Streptozotocin) (45 میلی‌گرم/کیلوگرم) انجام شد. تمرین استقامتی به مدت شش هفته اجرا شد. آزمون­های Tail-Flick و Von Frey برای سنجش پردردی حرارتی و آلوداینیای مکانیکی استفاده گردید. بررسی بیان سینتافیلین با روش ایمونوهیستوشیمی صورت گرفت. داده­ها توسط آنالیز واریانس دو راهه تجزیه و تحلیل شدند.
یافته ­ها: آزمون­های Tail-Flick و Von Frey کاهش معنی­داری در آستانه حس درد در گروه‌های دیابتی نسبت به غیردیابتی نشان دادند. پس از تزریق STZ، قندخون در گروه‌های دیابتی نسبت به غیردیابتی به صورت معنی­داری افزایش یافت (05/0p<). در پایان پژوهش، وزن گروه‌های دیابتی نسبت به غیردیابتی کاهش معنی­داری داشت (05/0p<). همچنین تفاوت معنی­داری در بیان سینتافیلین بین گروه‌های HT، DC و DT با گروه HC (001/0p=)، بین گروه‌های HT و DT با گروه DC (001/0p=) و بین گروه HT با گروه DT (002/0p=) وجود داشت.
نتیجه ­گیری: تمرین استقامتی موجب بهبود پاسخ­های درد نوروپاتیک و افزایش بیان سینتافیلین در نخاع موش­های مبتلا به نوروپاتی دیابتی شد. این یافته­ها می­تواند به عنوان یک شیوه درمانی برای عوارض نوروپاتی دیابتی استفاده شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Six Weeks of Endurance Training on Syntaphilin Protein Expression in Spinal Cord Tissue of Rats with Experimental Diabetic Neuropathy

نویسندگان [English]

  • A Kooshesh 1
  • MR Kordi 2
  • H Rajabi 3
  • AR Kazemi 4
1 PhD student, Dept of Exercise physiology, Kish International Campus, University of Tehran, Kish Island, Iran.
2 Associate Prof, Dept of Sports Physiology, Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran.
3 Prof, Dept of Sports Physiology, Faculty of Physical Education and Sports Sciences, Kharazmi University, Tehran, Tehran, Iran.
4 Associate Prof, Dept of Sports Science, Faculty of Literature and Humanities, Valiasr University, Rafsanjan, Iran.
چکیده [English]

Introduction: Neuropathy is a common complication of diabetes mellitus. Syntaphillin is involved in the anchoring of mitochondria to microtubules. The present study aimed to investigate the effect of six weeks of endurance training on Syntaphillin expression in spinal cord tissue of rats with experimental diabetic neuropathy.
Materials and Methods: 40 rats were randomly divided into four groups, diabetes control (DC), diabetes training (DT), healthy control (HC), and healthy training (HT). Induction of diabetes was performed by intraperitoneal injection of the Streptozotocin solution (45 mg/kg). Endurance training was performed for six weeks. Tail-Flick and Von Frey tests were used to measure thermal hyperalgesia and mechanical allodynia. The expression of Syntaphillin was evaluated by immunohistochemistry. The data were analyzed using two-way ANOVA.
Results: Teal-flick and von-Frey tests showed that pain threshold was significantly reduced in diabetic groups compared to non-diabetic groups (p<0.05). After STZ injection, blood glucose in diabetic groups increased significantly compared to non-diabetic groups (p<0.05). At the end of the study, the weight of diabetic groups decreased significantly compared to non-diabetic groups (p<0.05). In addition, there was a significant difference in the expression of Syntaphilin between HT, DC, and DT groups with HC group (p=0.001), between HT and DT groups with DC group (p=0.001) and between HT group and DT group (p=0.002).
Conclusion: Endurance training improves neuropathic pain responses and increases the Syntaphilin expression in the spinal cord of diabetic rats. These findings can be considered as a treatment for the complications of diabetic neuropathy.

کلیدواژه‌ها [English]

  • Diabetic neuropathy
  • Syntaphilin
  • Endurance training
  • Rat
  • Streptozotocin
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