اثر کاهش فعالیت بدنی از طریق خراش عصب نخاعی بر بیان ژن‌های درگیر در جریان کلسیم عضله نعلی موش‌های صحرایی پس از سه مدل تمرین ورزشی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، گروه علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه ولی‌عصر (عج)، رفسنجان، ایران.

2 استادیار، گروه بیهوشی، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان، ایران.

3 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی ، دانشگاه بیرجند، بیرجند، ایران.

4 کارشناس ارشد، مرکز تحقیقات فیزیولوژی، پژوهشکده نوروفارماکولوژی، دانشگاه علوم پزشکی کرمان، کرمان، ایران.

چکیده

مقدمه: اختلال در هموستاز کلسیم درون سلولی از طریق ژن­های STIM1/ORAI1 و MG29 در آتروفی عضلانی نقش دارد. هدف از پژوهش حاضر، تعیین اثر کاهش فعالیت بدنی از طریق خراش عصبی بر بیان ژن­های STIM1/ORAI1 و MG29 در عضله نعلی موش­های صحرایی بعد از سه مدل تمرین ورزشی بود.
مواد و روش­ ها: مطالعه حاضر از نوع تجربی بود که در دانشگاه علوم پزشکی کرمان در سال 1399 انجام شد. تعداد 32 سر موش صحرایی نژاد ویستار به روش تصادفی ساده (قرعه­کشی) به 4 گروه شم (sham-CCI)، تمرین استقامتی (CCI-E)، تمرین مقاومتی (CCI-S) و تمرین ترکیبی (CCI-CO) تقسیم شدند. مدت برنامه تمرینی، 6 هفته و 4 جلسه در هفته بود. اندازه‌گیری بیان ژن­ها با روش Real-Time PCR انجام شد. برای تجزیه و تحلیل داده­ها از آزمون آنالیز واریانس یک­طرفه و آزمون تعقیبی توکی استفاده شد.  
یافته­ ها: تفاوت معنی­داری در بیان ژن­های STIM1 (001/0=p، 98/134F=)، MG29 (001/0> p،12/245F=) و ORAI1 (001/0=p، 58/152F=) بین گروه­ها وجود داشت. بیان ژن STIM1 و MG29 در گروه CCI-CO نسبت به گروه sham-CCI و CCI-E به‌ طور معنی­داری بیشتر بود. همچنین، بیان ژن ORAI1 در گروه CCI-CO نسبت به گروه sham-CCI ، CCI-E و CCI-S بیشتر بود (05/0>p).
نتیجه­ گیری: نتایج نشان می­دهد که احتمالاً کاهش فعالیت بدنی به شکل خراش عصب نخاعی سبب اختلال در هموستاز کلسیم شده و از طرف دیگر تمرینات ورزشی ترکیبی بیان ژن­های درگیر در جریان کلسیم را افزایش داده و با اختلال در هموستاز کلسیم و در نتیجه آتروفی عضلانی مقابله می­کند. 

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Decreased Physical Activity Through Chronic Constriction Injury on Calcium Flux Gene Expression in Soleus Muscle of Wistar Rats After Three Exercise Models

نویسندگان [English]

  • AR Kazemi 1
  • Z Navidi 2
  • N Nekoie 3
  • S Salari 4
1 Associated prof, Dept of Physical Education, Faculty of Letters and Humanities, Vali E-Asr University, Rafsanjan, Iran.
2 Assistant prof, Dept of Anesthesiology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
3 Ph.D student, Dept of Exercise Physiology, Faculty of Physical Education, Birjand University, Birjand, Iran.
4 MSc,Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
چکیده [English]

Introduction: Calcium flux disorders due to STIM1, ORAI1 and MG29 may contribute to muscle atrophy. Therefore, This study aimed to determine the effect of decreased physical activity through Chronic Constriction Injury (CCI) on the expression of these genes in rat soleus muscle after three exercise training models.
Materials and Methods: The present study was an experimental study that was conducted in Kerman university of medical sciences in 2020. 32 eight-week-old rats were randomly divided into 4 groups: CCI (Sham-CCI), CCI-endurance training (CCI-E), CCI-strength training (CCI-S), and CCI-combined training (CCI-CO). The training program was of 6 weeks’ duration, 4 sessions per week. Real-time PCR was used to measure the mRNA expression of genes. To determine the significance of the differences between the variables, one-way ANOVA test ,and if necessary, tukey test, was used.
Results: The results of one-way ANOVA showed that there was a significant difference in STIM1 (F=134/98, p=0/001), MG29 (F=245/12, p<0/001) and ORAI1 (F=152/58, p=0/001) gene expression. Furthermore, tukey test results showed that STIM1 and MG29 gene expression in CCI-CO group was more than those in Sham-CCI and CCI-E groups. Also, ORAI1gene expression in CCI-CO group was more than those in Sham-CCI, CCI-E and CCI-S groups (p<0.05).     
Conclusion: The results showed that the decrease in physical activity through CCI leads to calcium hemostasis disorders. On the other hand, combined training increases gene expression of involving calcium flux, and decreases disorders of calcium  hemostasis and muscle atrophy.

کلیدواژه‌ها [English]

  • Resistance
  • Endurance
  • and Combined Training
  • muscle atrophy
  • Wistar rat
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