1 دانشگاه علوم پزشکی رفسنجان،رفسنجان،ایران
2 مرکز تحقیقات پزشکی ملکولی، دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان ، ایران
3 دانشکده پزشکی، دانشگاه علوم پزشکی رفسنجان، رفسنجان ، ایران
عنوان مقاله [English]
Introduction: OCT4 is one of the major genes for controlling cell renewal and is the core of the proteins that is known as stemness state.OCT4 encodes several variants, the most famous is OCT4B1.This variant has expressed in cancer cell lines and cancer tissues and has anti-apoptotic potency. Apoptosis has an important role in the pathogenesis of various diseases including cancer and autoimmune disorders. Apoptosis is regulated by different gene families; TRAF (TNF receptor-associated factor) is one of these families which consist of three genes (TRAF1, TRAF2 and TRAF3).There is little Information about the effect of OCT4B1 on gene expression of TRAF family; so, the aim of this study was to evaluate the effect of OCT4B1suppression on expression of TRAF family.
Material and Methods: cancer cell lines AGS (gastric adenocarcinoma), 5637 (bladder cancer) and U87MG (brain tumor) were cultured in the test and control groups with the same conditions and were transected after reaching the desired cell density, using specific siRNA OCT4B1 (test group) and scramble siRNA (control). Cellular RNA was extracted and cDNA were synthesized 48 hours after transfection. Gene expressions of interest (TRAF family) were estimated by PCR Array method and the results were analyzed by RT software.
Results: A gene expression alteration in studied in the three cancer cell lines were similar.
Conclusion: According to the results, OCT4B1 suppression inhibits the expression of TRAF family and this induces apoptosis in the cancer cell lines. Therefore, OCT4B1 can be considered in the recognition of mechanism of cancer and also cancer therapy.