مقایسه سه شدت متفاوت تمرینی بر بیان پروتئین LSDP5، سطوح سرمی گلوکز و انسولین رت‌های دیابتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم ورزشی، دانشگاه شهرکرد، شهرکرد، ایران

2 دانشیار، گروه علوم ورزشی، دانشگاه شهرکرد، شهرکرد، ایران

3 دانشیار، گروه علوم ورزشی، دانشگاه شهرکرد، شهرکرد، ایران.

4 استادیار، گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه شهرکرد، شهرکرد، ایران.

چکیده

مقدمه: اختلال متابولیسم چربی در ایجاد دیابت و مقاومت به انسولین در عضلات اسکلتی نقش دارد. از طرفی فعالیت بدنی بر لیپولیز درون عضلانی تاثیرگذار است. Lipid storage droplet protein 5 از پروتئین‌های مهم در تنظیم لیپولیز سلول‌های عضلانی است. هدف از این مطالعه مقایسه سه شدت متفاوت تمرینی بر بیان پروتئین LSDP5، سطوح سرمی گلوکز و انسولین رت‌های دیابتی است.
 مواد و روش‌ها‌: تعداد 32 سر رت نر نژاد ویستار به ‌صورت تصادفی به چهار گروه مساوی شامل سه گروه تمرینی (گروه تمرین استقامتی کم شدت، شدت متوسط و گروه با تمرین تداومی با شدت بالا) و یک گروه کنترل تقسیم شدند. پس از دیابتی کردن رت­ها با تزریق درون صفاقی استرپتوزوسین، تمرین استقامتی به مدت هشت هفته، سه جلسه در هفته اعمال شد. گروه کنترل مداخله‌ای دریافت نکردند. بیان نسبی LSDP5 با تکنیک Western blot انجام شد. معنی‌دار بودن تفاوت بین متغیرها توسط آزمون آماری تحلیل واریانس یک‌طرفه و آزمون تعقیبی توکی تعیین شد.
یافته‌ها: اختلاف معنی‌داری بین چهار گروه در متغیرهای LSDP5  مشاهده شد (03/0=p). تفاوت بین دو گروه کنترل دیابتی و گروه دیابتی تمرین با شدت بالا معنی­دار بود (019/0=p) اما تفاوت معنی‌داری در میزان LSDP5 در گروه تمرین کم شدت و شدت متوسط نسبت به گروه کنترل وجود نداشت (به ترتیب 64/0=p و  45/0=p).
نتیجه‌گیری: با توجه به نتایج می‌توان به تأثیر تمرینات استقامتی با شدت بالا بر افزایش بیان پروتئین LSDP5 در نمونه‌های دیابتی اشاره کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Survey on Comparison of three Different Training Intensities on Expression of LSDP5 Protein, Serum Levels of Glucose and Insulin in Diabetic Rats

نویسندگان [English]

  • M Ghafari 1
  • E Banitalabi 2
  • M Faramarzi 3
  • A Mohebi 4
1 Assistant Prof, Dept of Sport Sciences, Shahrekord University, Shahrekord, Iran.
2 Associate prof, Dept of Sport Sciences, Shahrekord University, Shahrekord, Iran.
3 Associate prof, Dept of Sport Sciences, Shahrekord University, Shahrekord, Iran.
4 Assistant prof, Dept of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University. Shahrekord, Iran.
چکیده [English]

Introduction: Lipid metabolism disorder plays an important role in diabetes and insulin resistance in skeletal muscles. On the other hand, intramuscular lipolysis can be affected by physical activities. Lipid storage droplet protein 5 (LSDP5) (is one of the important proteins involving in regulation of muscle lipolysis. Therefore, the aim of this study was to compare the three different training intensities on expression of LSDP5 protein, serum glucose and insulin levels in diabetic rats.
Materials and Methods: Thirty two male Wistar rats were randomly divided into four groups of 8 including three intervention groups;  with low- moderate- and  high-intensity endurance training and a control group. In all four groups, diabetes mellitus was induced by intraperitoneal injection of streptozotocin, 55 mg/kg and endurance training was applied three times a week for eight weeks and the control group received nothing. The expression of the LSDP5 protein was analyzed by Western blot technique. To determine the significance of differences between the groups, the results were analyzed using one-way ANOVA and Tukey post-hoc test.
Results: There was a significant difference was observed between four groups in LSDP5 variables (p = 0.03). There was a significant difference between diabetic controls group and high intensity group (p = 0.019), but there was no significant difference in LSDP5 in the low intensity and moderate intensity training group (respectively p = 0.64 and p = 0.45).
Conclusion: According to the results, high intensity endurance exercise increased protein expression LSDP5 in diabetic samples.

کلیدواژه‌ها [English]

  • Diabetes
  • LDSP5 protein
  • Endurance Training
  • Insulin Resistance

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