تأثیر هشت هفته تمرین مقاومتی با شدت متوسط بر محتوی و فعالیت آنزیم تلومراز بافت قلب در موش‌های صحرایی سالمند

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری،گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

2 استادیار، گروه فیزیولوژی ورزشی ، دانشکده علوم ورزشی، دانشگاه ارومیه، ارومیه، ایران.

3 دانشیار، گروه فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ارومیه، ارومیه، ایران.

چکیده

مقدمه: اختلالات فیزیولوژیکی و بیماری­های مرتبط با سالمندی خطر کاهش عملکرد فیزیکی را افزایش می­دهند. سبک زندگی غیر فعال با بروز نشانگرهای پیری سلولی مانند کاهش طول تلومر مرتبط است. عامل اصلی ثبات و افزایش طول تلومر آنزیم تلومراز است. هدف پژوهش حاضر تأثیر هشت هفته تمرین مقاومتی با شدت متوسط بر محتوی و فعالیت آنزیم تلومراز بافت قلب در موش­های صحرایی سالمند بود.
مواد و روش ­ها: این مطالعه از نوع تجربی بوده که در آن 20 سر موش صحرایی نر 46 هفته­ای با وزن 260 تا 310 گرم به صورت تصادفی در دو گروه 10 تایی شامل گروه تمرین و گروه کنترل قرار گرفتند. گروه تمرین به مدت هشت هفته و پنج روز در هفته تمرین مقاومتی با شدت متوسط انجام دادند. پس از اتمام دوره در حالت ناشتا و 48 ساعت بعد از آخرین جلسه تمرینی نمونه‌های بافت قلبی از موش­های صحرایی جمع‌آوری شد. برای اندازه­گیری فعالیت آنزیم تلومراز از روش PCR-ELISA و برای اندازه­گیری محتوی تلومراز از روش ساندویچ الایزا استفاده شد. داده­ها با استفاده از آزمون آماری t مستقل آنالیز شدند.
یافته ­ها: نتایج نشان داد که در گروه تمرینی نسبت به گروه کنترل، فعالیت آنزیم تلومراز (002/0=p) و محتوی آنزیم تلومراز (042/0=p) به صورت معنی­داری بیشتر بود.
نتیجه ­گیری: بر اساس نتایج پژوهش حاضر به نظر می­رسد که هشت هفته تمرین مقاومتی با شدت متوسط می­تواند سبب افزایش فعالیت و محتوی آنزیم تلومراز در بافت قلب موش­های صحرایی سالمند شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Eight Weeks of Moderate Intensity Resistance Training on Cardiac Telomerase Enzyme Content and Activity in Elderly Rats

نویسندگان [English]

  • Y Abdollahpour 1
  • M Zolfaghari 2
  • F Ghaderi Pakdel 3
1 PhD Student, Dept of Sport Sciences, Urmia University, Urmia, Iran.
2 Assistant Prof, Dept of Sport Sciences, Urmia University, Urmia, Iran.
3 Associate Prof of Physiology, Dept of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
چکیده [English]

Introduction: Age-related physiological disorders and diseases increase the risk of decline in physical functioning. Sedentary lifestyle is associated with the deterioration of cellular aging biomarkers such as telomere length shortening. Telomerase is the main factor for the stability and extension of telomere length. The aim of this study was to evaluate the effect of eight weeks of resistance training on cardiac telomerase enzyme content andactivity in elderly rats.
Materials and Methods: In this experimental study, 20 male Wistar rats (46 weeks of age) weighing 260 to 310 g were randomly assigned to two groups: the control group and the exercise group. The exercise group performed moderate intensity resistance exercise for 8 weeks, 5 days per week. Two weeks were considered for environment adaptation, biological rhythms and ladder familiarity. After two weeks, the major training protocol began, including three sessions of resistance training per week for eight weeks. 48 hours after the last training session, samples from the heart tissue were collected in fasting state. The PCR-ELISA method was used to measure the activity of telomerase, and the ELISA sandwich method was used to measure the telomerase content. Data were analyzed using an independent t-test.
Results: The results showed that the activity (p=0.002) and content (p=0.042) of telomerase enzyme in the training group was significantly higher than the control group.
Conclusion: According to the results of this study, it seems that eight weeks of moderate-intensity resistance training can increase the activity and content of telomerase enzyme in the heart tissue of aged rats.

کلیدواژه‌ها [English]

  • aging
  • Rats
  • resistance training
  • Telomerase
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