Community Health Journal

Community Health Journal

The survay of curcumin effect on the expressional profile of OCT4, Nanog and Nucleostemin genes in AGS (adenocarcinoma) cancer cell line

Authors
MR Mirzaei
M Mahmoodi
MR Hajizadeh
F Bagrezaei
V Akbarpoor
R Bahramabadi
Abstract
Introduction: Curcumin is the natural yellow pigment in turmeric isolated from the rhizome of the plant Curcuma longa. Curcumin inhibits formation and invasive cancer cells and destroys cancer cells resistant to chemotherapeutic drugs. The purpose of this study was the survey of effects of different concentrations of alcoholic curcumin on the OCT4, Nanog and Nucleostemin genes in the AGS (gastric adenocarcinoma) cell line.
Material and Methods: The AGS cell line was cultured in RPMI-1640 (Gibco), supplemented with penicillin/streptomycin (100 U/ml and 100 lg/ml, respectively) and 10% fetal bovine serum, at 37°C in a humidified atmosphere of 5% CO2. in 60–70% cell confluence, the cells were treated with Curcumin Cocentration (20,40, 100) μl and incubated for 24,48 and 72 hours. Finally, total RNA werecextracted and cDNA were synthesized and the expression of mentioned genes was detected.
Results: expression rate of OCT4A, OCT4B, NANOG and Nucleostemin (GLN3) at concentrations less than 20 µg/ml were reduced but OCT4B1 expression showed increased by hours respectively.
Conclusion: The results showed that curcumin inhibited cell division, also, this study could be the basis for more extensive studies on the anti-cancer effect of the combined plants.
Keywords
curcumin
AGS cell line
Gene expression