Community Health Journal

Community Health Journal

The Effects of an 8-Week Resistance-Interval Training on the Telomere Length, Telomerase Activity, and TRF2 Expression in Sedentary Young Men

Document Type : Original Article

Authors
y Mohammadnajad 1
H matinhomaee 2
MA Azabijani 3
1 PhD student, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
2 Assistant Prof, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
3 Prof, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
10.22123/chj.2018.120259.1106
Abstract
Introduction: Physical activities and increased physical fitness have always been recognized as a possible factor in the reduction of diseases and the related mortality. The telomere length is considered as a major cell age biomarker and is connected with diseases. The current study was conducted aimed at examining the effects of an 8-week resistance-interval training on the telomere length, telomerase activity, and TRF2 expression in sedentary young men.
Materials and Methods: In a semi-experimental experiment, 20 sedentary male students from the Islamic Azad University of Eslamshahr were selected and randomly assigned to the two groups of training (N=10) and control (N=10). The training protocol consisted of the concurrent interval and resistance trainings, with each session consisted of two steps. The first step was an interval training consisted of running on a treadmill, and the second one was related to a resistance training that was performed. Blood samples were taken half an hour before the first exercise and 24 hours after the last exercise, with 10 ml of blood taken from the brachial vein of each subject. Independent and dependent t-tests were used for the data analysis.
Results: The findings of present study demonstrated that an 8-week resistance-interval training prevented the telomere length shortening (t=3.87, p=0.022) and increased the telomerase activity (t=5.107, p=0.000). In addition, TRF2 levels (t=2.463.87, p=0.014) increased significantly compared to the pre-exercise condition.
Conclusion: The concurrent exercises increased the telomere length and telomerase activity of the subjects significantly. It seems that the exercises can have a favorable effect on telomere biology and the quality of life. The results indicated the importance of regular physical activities and consequently the reduced risk of diseases associated with aging.
Keywords
Resistance-interval training
Telomere
Telomerase
TRF2
  1. Aubert G, Lansdorp PM. Telomeres and aging. Physiological reviews 2008;88(2):557-79.
  2. Greider CW, Blackburn EH. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. cell 1985;43(2):405-13.
  3. Jones HJ, Janson SL, Lee KA. Leukocyte Telomere Length in Postmenopausal Women. JOGNN 2017;46(4):567-75.
  4. Borghini A, Giardini G, Tonacci A, Mastorci F, Mercuri A, Sposta SM, et al. Chronic and acute effects of endurance training on telomere length. Mutagenesis 2015;30(5):711-6.
  5. Choi ES, Chang YK, Lee DH, Ko J-H, Lim I, Bang H, et al. Gender-specific associations between quality of life and leukocyte telomere length. Maturitas 2018;107:68-70.
  6. Ornish D, Magbanua MJM, Weidner G, Weinberg V, Kemp C, Green C, et al. Changes in prostate gene expression in men undergoing an intensive nutrition and lifestyle intervention. Proceedings of the National Academy of Sciences 2008;105(24):8369-74.
  7. Hayflick L. The limited in vitro lifetime of human diploid cell strains. Experimental cell research 1965;37(3):614-36.
  8. Kong CM, Lee XW, Wang X. Telomere shortening in human diseases. The FEBS journal 2013;280(14):3180-93.
  9. Calado RT, Young NS. Telomere diseases. New England Journal of Medicine 2009;361(24):2353-65.
  10. Arsenis NC, You T, Ogawa EF, Tinsley GM, Zuo L. Physical activity and telomere length: impact of aging and potential mechanisms of action. Oncotarget 2017;8(27):45008-019.
  11. Donate LE, Blasco MA. Telomeres in cancer and ageing. PTRSLB: Biological Sciences 2011;366(1561):76-84.
  12. Eknoyan G. Adolphe Quetelet (1796–1874)—the average man and indices of obesity. Oxford University Press 2007;23:47-51.
  13. Ghafari H, Kazemi A, Alijanpour N.Accuracy of Regression Model Estimation of Maximum Oxygen Consumption Based on Non-Evidence Data in Female Students. JPEPA 2014;14:1091-100.] Persian]
  14. Puterman E, Lin J, Blackburn E, O'Donovan A, Adler N, Epel E. The power of exercise: buffering the effect of chronic stress on telomere length. PloS one 2010;5(5):e10837.
  15. Kazemi NS, Nabiuni M, Nouri ZS, Khosrojerdi F. Investigation of Telomerase activity and hTERT gene expression in MCF7 cells treated with papaverine. SUMSJ 2013; (20) 122-32.] Persian [ 
  16. Jin K. Modern biological theories of aging. Aging and disease 2010;1(2):72-4.
  17. Laufs U, Wassmann S, Czech T, Münzel T, Eisenhauer M, Böhm M, et al. Physical inactivity increases oxidative stress, endothelial dysfunction, and atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology 2005;25(4):809-14.
  18. Akbari H, Maleki MJ, Rawas'i AA, Kordi MR, Dzaji A, Miri SM, Maleki MH. Effect of a period of endurance training aimed at preventing cellular aging on the activity of cardiac telomerase enzymes and peripheral.blood lymphocytes in male rats. SJMCIRI 2013;31 (4): 315-28. ] Persian [
  19. Aguilera O, Fernández AF, Muñoz A, Fraga MF. Epigenetics and environment: a complex relationship. JAP 2010;109(1):243-51.
  20. Østhus IBØ, Sgura A, Berardinelli F, Alsnes IV, Brønstad E, Rehn T, et al. Telomere length and long-term endurance exercise: does exercise training affect biological age? A pilot study. PloS one 2012;7(12):e52769.
  21. Ludlow AT, Roth SM. Physical activity and telomere biology: exploring the link with aging-related disease prevention. JAR 2011;2011:1-12.
  22. Denham J, Nelson CP, O’Brien BJ, Nankervis SA, Denniff M, Harvey JT, et al. Longer leukocyte telomeres are associated with ultra-endurance exercise independent of cardiovascular risk factors. PloS one 2013;8(7):e69377.
  23. Saki B, Bahrami A, Ebrahim K, Abedi-Yekta A, Hedayati M. Effect of concurrent training on telomere length in patients with myocardial infarction: Randomised clinical trial of cardiac rehabilitation. Gene Reports 2016;4:264-8.
  24. Laye MJ, Solomon TP, Karstoft K, Pedersen KK, Nielsen SD, Pedersen BK. Increased shelterin mRNA expression in peripheral blood mononuclear cells and skeletal muscle following an ultra-long-distance running event. JAP 2011;112(5):773-81.
  25. Mason C, Risques RA, Xiao L, Duggan CR, Imayama I, Campbell KL, et al. Independent and combined effects of dietary weight loss and exercise on leukocyte telomere length in postmenopausal women. Obesity 2013;21(12):E549-E54.
  26. Cozzo C, Larkin J, Caton AJ. Cutting edge: self-peptides drive the peripheral expansion of CD4+ CD25+ regulatory T cells. The Journal of Immunology 2003;171(11):5678-82.
  27. Valdes AM, Andrew T, Gardner JP, Kimura M, Oelsner E, Cherkas LF, et al. Obesity, cigarette smoking, and telomere length in women. The lancet 2005;366(9486):662-4.