Community Health Journal

Community Health Journal

Survey on Comparison of three Different Training Intensities on Expression of LSDP5 Protein, Serum Levels of Glucose and Insulin in Diabetic Rats

Document Type : Original Article

Authors
M Ghafari 1
E Banitalabi 2
M Faramarzi 2
A Mohebi 3
1 Assistant Prof, Dept of Sport Sciences, Shahrekord University, Shahrekord, Iran.
2 Associate prof, Dept of Sport Sciences, Shahrekord University, Shahrekord, Iran.
3 Assistant prof, Dept of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University. Shahrekord, Iran.
10.22123/chj.2018.91614.1012
Abstract
Introduction: Lipid metabolism disorder plays an important role in diabetes and insulin resistance in skeletal muscles. On the other hand, intramuscular lipolysis can be affected by physical activities. Lipid storage droplet protein 5 (LSDP5) (is one of the important proteins involving in regulation of muscle lipolysis. Therefore, the aim of this study was to compare the three different training intensities on expression of LSDP5 protein, serum glucose and insulin levels in diabetic rats.
Materials and Methods: Thirty two male Wistar rats were randomly divided into four groups of 8 including three intervention groups;  with low- moderate- and  high-intensity endurance training and a control group. In all four groups, diabetes mellitus was induced by intraperitoneal injection of streptozotocin, 55 mg/kg and endurance training was applied three times a week for eight weeks and the control group received nothing. The expression of the LSDP5 protein was analyzed by Western blot technique. To determine the significance of differences between the groups, the results were analyzed using one-way ANOVA and Tukey post-hoc test.
Results: There was a significant difference was observed between four groups in LSDP5 variables (p = 0.03). There was a significant difference between diabetic controls group and high intensity group (p = 0.019), but there was no significant difference in LSDP5 in the low intensity and moderate intensity training group (respectively p = 0.64 and p = 0.45).
Conclusion: According to the results, high intensity endurance exercise increased protein expression LSDP5 in diabetic samples.
Keywords
Diabetes
LDSP5 protein
Endurance Training
Insulin Resistance
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