اثر 8 هفته تمرین مقاومتی همراه با مکمل‌گیری تائورین بر میزان گلوکز ناشتا، مقاومت به انسولین و GLUT4در بافت عضله نعلی موش‌های صحرایی نر ویستار مبتلا به دیابت نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد اسلامشهر، اسلامشهر، ایران.

2 استادیار، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد اسلامشهر، اسلامشهر، ایران.

3 دانشیار،گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد اسلامشهر، اسلامشهر، ایران.

4 استادیار، گروه مدیریت ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد اسلامشهر، اسلامشهر، ایران.

چکیده

مقدمه: دیابت نوع 2 یک بیماری اختلال متابولیک است. اخیراً برخی تحقیقات نشان داده­اند، احتمالاً اسیدآمینه تائورین می­تواند در پیش­گیری و بهبود عوارض برخی از بیماری­های متابولیک مؤثر باشد. هدف مطالعه حاضر، تعیین اثر تمرین­مقاومتی همراه با تائورین بر میزان گلوکز ­ناشتا، مقاومت­ انسولین و GLUT4، در بافت عضله نعلی موش­های دیابتی نوع 2 بود.
مواد و روش ­ها: در این مطالعه تجربی، 40­ سر موش نر ویستار با دامنه وزنی 215 الی 230 گرم به­صورت تصادفی در 5 گروه 8 سری: کنترل­ سالم، کنترل­ دیابتی، ­تائورین، ­تمرین­مقاومتی و ­تمرین­­مقاومتی همراه با تائورین تقسیم شدند. جهت القای دیابت نوع 2 از روش High Fat Diet-Streptozotocin استفاده شد. تمرین­­مقاومتی به مدت 8 هفته، هر هفته 5 جلسه با شدت30 تا 100 درصد وزن بدن اجرا شد. 250 میلی­گرم تائورین به ازای هر کیلوگرم وزن بدن در دو گروه ­تائورین و تمرین­ همراه با تائورین استفاده شد. از آزمون تحلیل واریانس یک­راهه برای تجزیه و تحلیل نتایج استفاده گردید.
یافته­ ها: القای دیابت سبب کاهش (4‎(GLUT، افزایش گلوکز و مقاومت­انسولین در نمونه­های دیابتی گردید. 8 هفته تمرین مقاومتی، مکمل­گیری تائورین و تمرین ­مقاومتی همراه با تائورین سبب کاهش معنی­دار گلوکز ­ناشتا (90/2896F=، 0040/0p=)، مقاومت­انسولین (43/1471F=، 0010/0p=)، و افزایش محتوای 4GLUT (18/1936F=، 0020/0p=)، گردید. بین گروه­های ­تمرین­مقاومتی و ­تائورین تفاوت معنی­داری در میزان تغییرات مشاهده نشد. گروه تمرین­ همراه با تائورین تفاوت معنی­داری در میزان تغییرات در مقایسه با دو گروه تجربی دیگر داشت.
نتیجه­ گیری: ترکیب تمرین با تائورین می­تواند در طی 8 هفته، اثرات مفیدی بر متغیرهای این تحقیق در بیماران مبتلا به دیابت نوع 2 داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of 8 Weeks of Resistance Training with Taurine Supplementation on Fasting Glucose, Insulin Resistance, and GLUT4 in Soleus Muscle Tissue in Male Wistar Rats with Type 2 Diabetes

نویسندگان [English]

  • F Khavarian 1
  • Y Kazemzadeh 2
  • M Hajirasouli 3
  • S Mirzayan Shanjani 2
  • S Sedaghati 4
1 Ph.D Student, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran.
2 Assistant Prof, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran.
3 Associate Prof, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran.
4 Assistant Prof, Dept of Sports Management, Faculty of Physical Education, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran.
چکیده [English]

Introduction:Type 2 diabetes is a metabolic disorder. Recently, some studies have shown that the amino acid taurine may be effective in preventing and improving the effects of some metabolic diseases. This study aims to determine the effect of resistance training with taurine supplementation on fasting glucose, insulin resistance, and GLUT4 in soleus muscle tissue of type2 diabetic mice.
Materials and Methods: In this experimental study, forty male Wistar rats, weighing 215 to 230 g, were randomly divided into 5 groups of 8: healthy control, diabetic control, taurine, resistance training, and resistance training with taurine. HFD-STZ (High Fat Diet-Streptozotocin) method was used to induce type 2 diabetes. Resistance training was performed 5 sessions per week for 8 weeks, with an intensity of 30% to 100% of body weight. 250 mg of taurine per kg of body weight was used in two groups of taurine and exercise with taurine. One-way analysis of variance was used in the research.
Results: Induction of diabetes resulted in decreased (GLUT4), increased glucose, and insulin resistance in diabetic specimens. 8 weeks of resistance training, taurine supplementation, and resistance training with taurine caused a significant reduction in fasting glucose (F=2896.90, p=0.4040), insulin resistance (F=1471.43, p=0.0010), and an increase in GLUT4 content (F=1936.18, p=0.0020). There was no significant difference in the changes between the two diabetic groups of exercise and taurine. However, the exercise group with taurine had a significant difference in the rate of change compared to the other two experimental groups.
Conclusion: Combining exercise with taurine (for 8 weeks) can have beneficial effects on the variables of this study in patients with type 2 diabetes.

کلیدواژه‌ها [English]

  • Type 2 diabetes
  • GLUT4
  • insulin resistance
  • fasting glucose
  • taurine
  • Rat
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