تأثیر هشت هفته تمرین مقاومتی ـ تناوبی بر طول تلومر، فعالیت تلومراز و میزان بیان پروتئین متصل به تلومر مردان جوان غیر فعال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران.

2 دانشیار، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران.

3 استاد، گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد تهران مرکزی، تهران، ایران.

چکیده

مقدمه: فعالیت ورزشی و افزایش آمادگی بدنی به عنوان یک عامل احتمالی در کاهش بیماری‌ها و مرگ­و­میر ناشی از آن شناخته ­شده ­است. طول تلومر یک بیومارکر اصلی سن سلول به شمار می­رود و با بیماری­ها ارتباط دارد. هدف از پژوهش حاضر بررسی تأثیر هشت هفته تمرین  مقاومتی-تناوبی بر طول تلومر، فعالیت تلومراز و میزان بیان پروتئین متصل به تلومر (TRF2) بود.
مواد و روش‌ها: در این کارآزمایی نیمه تجربی، تعداد 20 مرد جوان غیرفعال از دانشجویان دانشگاه آزاد اسلامی واحد اسلامشهر در سال تحصیلی 96-95 انتخاب و به طور تصادفی به دو گروه مساوی تمرین و کنترل تقسیم شدند. پروتکل تمرینی شامل تمرین هم‌زمان مقاومتی و تناوبی بود. مرحله اول، تمرین تناوبی دویدن بر روی نوار گردان و مرحله دوم اجرای تمرین مقاومتی بود. نیم ساعت قبل از تمرین و 24 ساعت بعد از آخرین تمرین، 10 میلی­لیتر خون از ورید بازویی آزمودنی­ها گرفته شد. برای تجزیه و تحلیل داده­ها از روش آماری  t مستقل و زوجی استفاده شد.
یافته ­ها: یافته­ها نشان‌ داد هشت هفته تمرین مقاومتی­-تناوبی مانع از کوتاه شدن طول تلومر (87/3 , t= 022/0p=) و باعث افزایش فعالیت تلومراز (107/5 , t= 000/0p=) می‌شود. همچنین در مقایسه با قبل از تمرین، افزایش معنی­دار مقادیر پروتئین متصل به تلومر (463/2 t= ، 014/0p=) دیده شد.
نتیجه­ گیری: تمرین ترکیبی باعث افزایش طول تلومر و افزایش فعالیت تلومراز آزمودنی­ها گردید. به نظر می­رسد این تمرین تأثیر مطلوبی بر بیولوژی تلومر داشته باشد. نتایج، اهمیت فعالیت ورزشی منظم و کاهش خطر ابتلا به بیماری­های مرتبط با افزایش سن را نشان داد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effects of an 8-Week Resistance-Interval Training on the Telomere Length, Telomerase Activity, and TRF2 Expression in Sedentary Young Men

نویسندگان [English]

  • y Mohammadnajad 1
  • H matinhomaee 2
  • MA Azabijani 3
1 PhD student, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
2 Assistant Prof, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
3 Prof, Dept of Exercise Physiology, Faculty of Physical Education, Islamic Azad University, Central Tehran Branch, Tehran, Iran.
چکیده [English]

Introduction: Physical activities and increased physical fitness have always been recognized as a possible factor in the reduction of diseases and the related mortality. The telomere length is considered as a major cell age biomarker and is connected with diseases. The current study was conducted aimed at examining the effects of an 8-week resistance-interval training on the telomere length, telomerase activity, and TRF2 expression in sedentary young men.
Materials and Methods: In a semi-experimental experiment, 20 sedentary male students from the Islamic Azad University of Eslamshahr were selected and randomly assigned to the two groups of training (N=10) and control (N=10). The training protocol consisted of the concurrent interval and resistance trainings, with each session consisted of two steps. The first step was an interval training consisted of running on a treadmill, and the second one was related to a resistance training that was performed. Blood samples were taken half an hour before the first exercise and 24 hours after the last exercise, with 10 ml of blood taken from the brachial vein of each subject. Independent and dependent t-tests were used for the data analysis.
Results: The findings of present study demonstrated that an 8-week resistance-interval training prevented the telomere length shortening (t=3.87, p=0.022) and increased the telomerase activity (t=5.107, p=0.000). In addition, TRF2 levels (t=2.463.87, p=0.014) increased significantly compared to the pre-exercise condition.
Conclusion: The concurrent exercises increased the telomere length and telomerase activity of the subjects significantly. It seems that the exercises can have a favorable effect on telomere biology and the quality of life. The results indicated the importance of regular physical activities and consequently the reduced risk of diseases associated with aging.

کلیدواژه‌ها [English]

  • Resistance-interval training
  • Telomere
  • Telomerase
  • TRF2

مراجع

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